Human Complement Component C5a Protein, Fc Tag

Source : 

Human C5a, Fc Tag (C5A-H525a) is expressed from human 293 cells (HEK293). It contains AA Leu 679 - Arg 751 (Accession # P01031-1).

 

Molecule : Complement Component C5a

 

Synonyms : C5a,Complement Component 5a

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : XP_015292262.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 34.6 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

 

References :

(1) Gerard, C. and N.P. Gerard, 1994, Annu. Rev. Immunol.12:775.
(2) Monk, PN. et al., 2007, British Journal of Pharmacology. 152: 429-48.
(3) Reis ES, et al., 2011,Brain Behav Immun. Apr 23.
(4) Bergseth G,et al., 2011, Ann Thorac Surg., 91(2):527-33.
(5) Manthey HD,et al., 2009, Int J Biochem Cell Biol., 41(11):2114-7.