Human Complement Component C2 ELISA Kit

分子 : C2

 

抗原貨號 : 10154-H08H

 

產品介绍 : For the quantitative determination of Human Complement Component C2 concentration in serum, or cell culture supernates.

 

線性範圍: 234.38-15000

 

包被抗體類型 : rabbit mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 110.77

 

天然樣本測定含量: The average concentration of C02 in 10 normal human serum is 47.86 +/- 14.60 μg/mL ranging from 21.43 to 67.86 μg/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural CO2, and measured 252.51 ng/mL.

 

特異性 : This assay recognizes both recombinant and natural Human CO2. Human C1S and C5a were prepared at 300 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 :

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution