Human COMP / TSP5 ELISA Pair Set

分子 : COMP

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

 

 

線性範圍 : 39.06-2500

 

包被抗體 : 0.5 mg/mL of mouse anti-COMP monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10173-MM02)

 

檢測抗體: Each vial contains 120 μg biotinylated rabbit anti-COMP polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.

 

標準品 : Each vial contains 60 ng of recombinant COMP. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.

 

生物素交聯HRP: 50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.

 

靈敏度 : The minimum detectable dose of human COMP / TSP5 was determined to be approximately 39.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The human COMP / TSP5 ELISA Pair Set is for the quantitative determination of human COMP.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for COMP /TSP5 coated on a 96-well plate. Standards and samples are added to the wells,and any COMP / TSP5 present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-COMP polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of COMP / TSP5 present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.

 

檢測限: 39.1 pg/ml

 

血清樣本檢測範圍 :