Human Chorionic Gonadotropin (HCG-free beta) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Anti-Beta HCG Coated Strip plates (96 wells). Cat.# 411 1 Plate 2. Std. A, Cat. # 412, 0 mIU/ml (sample diluent), 11 ml 1 vial 3. Std. B, Cat. # 413, 2.5 mIU/ml, 0.3 ml 1 vial 4. Std. C, Cat. # 414, 5 mIU/ml, 0.3 ml 1 vial 5. Std. D, Cat. # 415, 10 mIU/ml, 0.3 ml 1 vial 6. Std. E, Cat. # 416, 30 mIU/ml, 0.3 ml 1 vial 7. Std. F, Cat. # 417, 100 mIU/ml, 0.3 ml 1 vial Standards are provided in BSA-containing diluent and calibrated against WHO IRP 75/551 (1 mIU/ml=1 ng/mL). 8. Free beta Low Control Serum (see conc on the vial) 410C1 9. Free beta High Control Serum (see conc on the vial) 410C2 10. Wash Buffer (20X), 50 ml WB-20 11. Anti-free-beta HCG-HRP Conjugate,Cat # 418, 11 ml ready-to-use 1 bottle 12. HRP Substrate Solution A, 11 ml Cat. # 410SA 13. HRP Substrate Solution B, 11 ml Cat. # 410SB 14. Stop solution, Cat. # T-10, 10 ml 1 bottle 15. Complete Instruction Manual, M410 1

Form

Stds=0-100 mIU/ml ; Sample=25 ul; Sensitivity= 0.5 mIU/ml; 40 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

Human free-HCG ELISA kit is specific for free-beta subunit of HCG with no significant reactivity with HCG (intact), alpha-HCG, LH, TSH, FSH or other peptide hormones. Human free-HCG ELISA kit species crossreactivity with HCG from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates