Human CDNF ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

CDNF

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of human CDNF protein in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : CDNF is a trophic factor for dopamine neurons. It prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1704

 

Description (Long Description) : 

The human CDNF kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human CDNF pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human CDNF concentration are added to these wells and allowed to complex with the bound antibody to human CDNF. A biotinylated polyclonal goat antibody to human CDNF is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CDNF in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant human CDNF expressed in an E.coli expression system; Immunogen sequence: Q25-L187

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, human CDNF protein standards, human CDNF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1704