Human CDC2 & CCNE1 Heterotrimer Protein_CT027-H0907B

分子 : CDC2 & CCNE1

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.

纯度: > 90 % as determined by SDS-PAGE

内毒素 : < 1.0 EU per μg protein as determined by the LAL method.

預測N端 : Met & His

缓冲液 : Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.

NP號 : NP_001777.1 & NP_001229.1

氨基酸序列 : Met1-Met297 & Met1-Ala410

標籤 : N-GST & N-His