Human CD99 / MIC2 Protein, His Tag

Source : 

Human CD99, His Tag (CD9-H5221) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # AAH02584).

 

Molecule : CD99

 

Synonyms : CD99,HBA71,MIC2,MIC2X,MIC2Y,MSK5X,12E7

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH02584

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 10.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 10.9 kDa. The protein migrates as 25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

 

References :

(1) Gelin C., et al., 1989, EMBO J. 8:3253-3259.
(2) Aussel C., et al., 1993, Biochemistry 32 (38): 10096–101.
(3) Dworzak MN., et al., 1994, Blood 83 (2): 415–25.
(4) Bernard G., et al., 1997, Journal of immunology (Baltimore, Md. : 1950) 158 (6): 2543–50.