Human CD86 / B7-2 ELISA Pair Set

分子 : CD86

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 3.13-200

 

包被抗體 : 1 mg/mL of rabbit anti-Human CD86 / B7-2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10699-R103)

 

檢測抗體: 0.2 mg/mL of rabbit anti-Human CD86 / B7-2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10699-R118)

 

標準品 : Each vial contains 42 ng of recombinant Human CD86 / B7-2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of Human CD86 / B7-2 was determined to be approximately 3.13 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The Human CD86 / B7-2 ELISA Pair Set is for the quantitative determination of Human CD86 / B7-2.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CD86 / B7-2 coated on a 96-well plate. Standards and samples are added to the wells, and any Human CD86 / B7-2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human CD86 / B7-2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CD86 / B7-2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

 

檢測限: 3.13 pg/ml

 

血清樣本檢測範圍 :