Human CD8 alpha / CD8A Protein, His Tag

Source : 

Human CD8 alpha, His Tag (CDA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).

 

Molecule : CD8 alpha

 

Synonyms : CD8A,CD8,Leu2,MAL,p32

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_001759.3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 19.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

 

References :

(1) Zhu J, et al. Nature. 2013. 497(7450):494-7.
(2) Gibbings DJ, et al. BMC Immunol. 2007. 8:12.
(3) Addison EG, et al. Immunology. 2005. 116(3):354-61.