Human CD74 / DHLAG Protein, His Tag

Source : 

human CD74, His Tag (CD4-H524c) is expressed from human 293 cells (HEK293). It contains AA Gln 73 - Met 232 (Accession # NP_004346.1).

 

Molecule : CD74

 

Synonyms : CD74,DHLAG,HLADG,II,Ia-GAMMA,p33,CLIP,INVG34

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_004346.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 19.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Cluster of Differentiation 74 (CD74) is also known as HLA class II histocompatibility antigen gamma chain (HLADG), DHLAG, Ia-GAMMA, invariant chain (Abbreviated Ii). CD74 / HLADG is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.

 

References :

(1) Cresswell P., 1994, Annu. Rev. Immunol. 12: 259–93.
(2) Charest A., et al., 2003, Genes Chromosomes Cancer 37:58-71.
(3) Leng L., et al., 2003, J. Exp. Med. 197:1467-1476.