Human CD5 Protein, His Tag

Source : 

Human CD5, His Tag (CD5-H52H5) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # NP_055022).

 

Molecule : CD5

 

Synonyms : CD5,LEU1

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_055022

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 40.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 45-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

 

References :

(1) van de Velde H., et al., 1991, Nature 351:662-665.
(2) Brill L.M., et al., 2004, Anal. Chem. 76:2763-2772.
(3) Rodamilans B., et al., 2007, J. Biol. Chem. 282:12669-12677.