Human CD47 Protein, Llama IgG2b Fc Tag, low endotoxin

Source : 

Human CD47, Llama IgG2b Fc Tag, low endotoxin (CD7-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).

 

Molecule : CD47

 

Synonyms : CD47,MER6,IAP,OA3

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : NP_942088.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 41.6 kDa

 

Characteristics :

This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.01 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

 

References :

(1) Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
(2) Latour S., et al., 2001, J. Immunol. 167:2547-2554.
(3) Piccio L., et al., 2005, Blood 105:2421-2427.