Human CD40 / TNFRSF5 Protein, Mouse IgG2a Fc Tag, low endotoxin

Source : 

Human CD40, Mouse IgG2a Fc Tag, low endotoxin (CD0-H525a) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).

 

Molecule : CD40

 

Synonyms : CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

 

Format : Powder

 

Category : Immune Checkpoint Proteins

 

Accession : NP_001241.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 46.1 kDa

 

Characteristics :

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.1 kDa. The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.1 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

 

Description :

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines.

 

References :

(1) Banchereau, J. et al., 1994, Annu. Rev. Immunol. 12: 881-922.
(2) Ni, C.Z. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 10395-10399.
(3) Ferrari.S. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 12614-12619.
(4) Bhushan, A and Covey, L.R. 2001, Immunol. Res. 24: 311-324.
(5) Armitage, R.J. et al., 1993, J. Immunol. 150:3671.