Human CD4 / LEU3 Protein, His Tag

Source : 

Human CD4, His Tag (LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782).

 

Molecule : CD4

 

Synonyms : CD4,CD4mut,LEU3

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH25782

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 41.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

 

References :

(1) Crise B., et al.,1990, J. Virol. 64:5585-5593.
(2) Lusso P., et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91:3872-3876.
(3) Zeitlmann L., et al., 2001, J. Biol. Chem. 276:9123-9132.