Human CD36 / SR-B3 Protein, His Tag

Source : 

Human CD36, His Tag (CD6-H5221) is expressed from human 293 cells (HEK293). It contains AA Gly 30 - Asn 439 (Accession # NP_001001547.1).

 

Molecule : CD36

 

Synonyms : CD36,SCARB3,BDPLT10,CHDS7,FAT,GP3B,GP4,GPIV,PASIV,Platelet Glycoprotein 4,glycoprotein IV,gpIV,glycoprotein IIIb,gpIIIb

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_001001547.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 48.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

 

References :

(1) Husemann, J. et al., 2002, Glia 40:195.
(2) Armstrong, L and P. Bornstein, 2003, Matrix. Biol. 22:63.
(3) Collins RF, et al., 2009, J. Biol. Chem. 284 (44): 30288–97.
(4) Stewart CR, et al., 2010, Nat. Immunol. 11 (2): 155–61.