Human CD31 / PECAM-1 Protein, His Tag

Source : 

Human CD31, His Tag (CD1-H5224) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Lys 601 (Accession # P16284-1).

 

Molecule : CD31

 

Synonyms : PECAM1,CD31,FLJ34100,FLJ58394

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_000433.4

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 66.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 66.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Cluster of Differentiation 31 (CD31) is also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

 

References :

(1) Newman PJ, Newman DK., 2004, Arterioscler. Thromb. Vasc. Biol. 23 (6): 953–64.
(2) Kalinowska A, Losy J., 2007, Eur. J. Neurol. 13 (12): 1284–90.
(3) Almendro N, et al., 2007, J. Immunol. 157 (12): 5411–21.