Human CD3 epsilon Protein, Strep Tag

Source : 

Human CD3E, Strep Tag (CDE-H5286) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).

 

Molecule : CD3 epsilon

 

Synonyms : FLJ18683,T3E,TCRE,CD3E,CD3-epsilon

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : NP_000724.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 14.8 kDa

 

Characteristics :

This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 14.8 kDa. The protein migrates as 16-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

 

References :

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) de Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10): 1512–7.
(4) Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1): 69–73.