Human CD14 Protein, His Tag

Source : 

Human CD14, His Tag (CD4-H5228) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Met 344 (Accession # AAH10507).

 

Molecule : CD14

 

Synonyms : CD14

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH10507

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 35.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.9 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

 

References :

(1) Kirkland TN, Viriyakosol S, et al., 1998, Prog. Clin. Biol. Res. 397: 79–87.
(2) Kitchens RL., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 61–82.
(3) Tapping RI, Tobias PS., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 108–21.
(4) Thomas, Celestine J., et al., 2002, FEBS Lett. (Netherlands) 531 (2): 184–8.
(5) Yu B, Wright S D., 1995, J. Inflamm. (UNITED STATES) 45 (2): 115–25.