Human CCL5/RANTES ELISA Kit

分子 : CCL5

 

抗原貨號 : 10900-HNAE

 

產品介绍 : For the quantitative determination of Human CCL5/RANTES concentration in serum, cell culture supernates and plasma.

 

線性範圍: 0.78-50

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: .24

 

天然樣本測定含量: Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural CCL5, and measured 3.87 ng/mL.The average concentration of Human CCL5 in 10 normal human serum is 19.46 +/- 5.21 ng/mL ranging from 12.65 to 30.35 n

 

特異性 : This assay recognizes both recombinant and natural Human CCL5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant Human CCL5 control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution