Human CBLB / RNF56 Protein, His Tag

Source : 

Human CBLB, His Tag (CBB-H5148) is expressed from E.coli cells. It contains AA Ala 39 - Phe 426 (Accession # Q13191-1).

 

Molecule : CBLB

 

Synonyms : CBL-B,CBLB,RNF56

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_733762.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 47.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.0 kDa. The protein migrates as 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 40 mM HEPES, 120 mM NaCl, pH7.5

 

Description :

Casitas B-lineage lymphoma proto-oncogene b (CBLB) is also known as RNF56. E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle.

 

References :

(1) Xiao Y., et al., 2015, J Immunol., 195 (9): 4218-4227.
(2) Desai SS., et al., 2015, J Biol Chem., 290 (42): 25595-25608.
(3) Van de Veerdonk FL., et al., 2016, Nat Med., 22 (8): 834-835.