Human Cathepsin B / CTSB Protein, His Tag

Source : 

Human Cathepsin B, His Tag (CTB-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)).

 

Molecule : Cathepsin B

 

Synonyms : CTSB,CPSB,APPS

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_001899.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 36.7 kDa & 29.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus.
The Human Cathepsin B will be further processed into mature form (Leu 80-Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 42-50

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH8.0

 

Description :

Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

 

References :

(1) Basrur V., et al., 2003, J. Proteome Res. 2:69-79.
(2) Chi A., et al., 2006, J. Proteome Res. 5:3135-3144.
(3) Royer-Zemmour B., et al., 2008, Hum. Mol. Genet. 17:3617-3630.
(4) Cao L., et al., 1994, Gene 139 (2): 163–9.