Human C5a ELISA Pair Set

分子 : C5

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 23.44-1500

 

包被抗體 : 1 mg/mL of mouse anti-Human C5a monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10604-MM08)

 

檢測抗體: 0.2 mg/mL of mouse anti-Human C5a monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10604-MM32)

 

標準品 : Each vial contains 18 ng of recombinant Human C5a. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of Human C5a was determined to be approximately 23.44 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The Human C5a ELISA Pair Set is for the quantitative determination of Human C5a.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human C5a coated on a 96-well plate. Standards and samples are added to the wells, and any Human C5a present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human C5a monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human C5a present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

 

檢測限: 23.44 pg/ml

 

血清樣本檢測範圍 :