Human C-peptide ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized.

KitContents

1. Anti-C-Peptide IgG coated microwell strip plate (96 wells), Cat. # 041 1 plate 2. Human C-Peptide Std. A (sample diluent), 11 ml, 0 ng/ml, Cat. # 042 1 vial 3. Human C-Peptide Std. B, 0.75 ml, 0.5 ng/ml, Cat. # 043 1 Vial 4. Human C-Peptide Std. C, 0.75 ml; 2.5 ng/ml, Cat. # 044 1 Vial 5. Human C-Peptide Std. D, 5.0 ng/ml, 0.75 m, Cat. # 045 1 Vial 6. Human C-Peptide Std. E, 10 ng/ml, 0.75 ml, Cat. # 046 1 Vial 7. Human C-Peptide Std. F, 20 ng/ml, 0.75 ml, Cat. # 047 1 Vial 8. Note: the Stds are not stable and should be stored in suitable aliquot at ?20oC for extended storage. Once vial is opened, the stds are stable for 5 days at 4oC. 9. Anti-C-Peptide-HRP Conjugate, 11 ml, Cat. # 048 1 bottle 10. HRP substrate Solution A, Cat. # 40SA 1 bottle 11. HRP substrate Solution B, Cat. # 40SB 1 bottle 12. Wash Buffer (20X), 50 ml WB-20 13. Stop solution, 10 ml, Cat. # T-10 1 bottle 14. Instruction Manual, M-0040 1

Form

Stds=0-20 ng/ml ; Sample=25 ul; Sensitivity= .0.3 ng/ml; 45 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

ADI C-peptide ELISA kit was tested with the following: Human Pro-insulin 23-33 split (63%), Human Pro-insulin 64-65 split (87%), Human insulin (0%), Human C-peptide of Insulin (100%). Fasting concentration of intact and split pro-insulin are typically only 1-2% of C-peptide. Crossreactivity with these molecules is not clinically significant. Human C-peptide ELISA kit species crossreactivity with C-peptide from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates