Human Bone Morphogenic Protein 7 (BMP-7) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Plasma, Urine, Culture medium

KitContents

1. Anti-Human BMP-7 coated strip plate (8 wells x 12 strips) 100-1912. Human BMP-7 Std. A (0 ng/ml), 0.2 ml 100-1923. Human BMP-7 Std. B (0.5 ng/ml), 0.2 ml 100-1934. Human BMP-7 Std. C (1 ng/ml), 0. 2 ml 100-1945. Human BMP-7 Std. D (2 ng/ml), 0. 2 ml 100-1956. Human BMP-7 Std. E (4 ng/ml), 0. 2 ml 100-1967. Human BMP-7 Std. F (6 ng/ml), 0. 2 ml 100-1978. Human BMP-7 Std. G (8 ng/ml), 0.2 ml 100-1989. (20x) Sample Diluent, 10ml SD-2010. Wash Buffer (100X), 10 ml WB-10011. (100x) Anti-Human BMP-7 Detection Ab, 0.12 ml 100-12812. (100x) Streptavidin-HRP conjugate, 0.12ml S-HRP10013. TMB Substrate, 12 ml 8009114. Stop solution, 12 ml 8010115. Instruction Manual M-100-190-B7H

Form

Stds=100-1600 pg/ml ; Sample=100 ul; Sensitivity= 20 pg/ml; 195 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

BMP-7 recombinant protein was detected in buffered solutions with proteins such as culture medium. There was no detectable protein in a limited testing of 20 human sera. Human BMP-7 ELISA kit cross-reactivity was tested with the following recombinant human proteins at 50ng/ml: BMP-2, BMP-3, BMP-4 BMP-5, BMP-6, BMP-8, TGF-ß1, TGF-ß2, TGF-ß3. All exhibited no cross-reactivity or interference.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates