Human Betacellulin Protein, Fc Tag

Source : 

Human Betacellulin, Fc Tag (BEN-H525x) is expressed from human 293 cells (HEK293). It contains AA Asp 32 - Tyr 111 (Accession # AAH11618).

 

Molecule : Betacellulin

 

Synonyms : betacellulin,BTC

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH11618

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 35.8 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 35.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Probetacellulin can be cleaved into the Betacellulin (BTC) that is a single-pass type I membrane protein containing 1 EGF-like domain. Predominantly expressed in pancreas and small intestine,Betacellulin (BTC) is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. As a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells, Betacellulin (BTC) is also the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Betacellulin (BTC) was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. The amino acid sequence of mature mBTC is 82.5%, identical with that of human BTC (hBTC), and both exhibit significant overall similarity with other members of the EGF family.

 

References :

(1) Watanabe T., et al., 1994, J. Biol. Chem. 269:9966-9973.
(2) Seno M., et al., 1996, Growth Factors 13:181-191.
(3) Bermingham Y., et al., 1996, Oncogene 12:345-353.