Human beta 2-Microglobulin / B2M Protein, His Tag

Source : 

Human B2M, His Tag (B2M-H5225) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (Accession # P61769-1).

 

Molecule : beta 2-Microglobulin

 

Synonyms : B2M

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH32589.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 12.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 12.6 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

β2 microglobulin is also known as Beta-2-microglobulin (B2M), is a component of MHC class I molecules which belongs to the beta-2-microglobulin family. B2M is present on all nucleated cells (excludes red blood cells). B2M associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. An additional function of B2M is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the cytoplasmic membrane of enterocytes and macrophages for degradation resulting in decreased iron uptake from food and iron release from recycled red blood cells respectively. Loss of this function causes iron excess and hemochromatosis. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP).

 

References :

(1) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.
(2) Gorevic P.D., et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83:7908-7912.
(3) Argiles A., et al., 1992, Nephrol. Dial. Transplant. 7:1106-1110.
(4) Momoi T., et al., 1995, Clin. Chim. Acta 236:135-144.
(5) Collins E.J., et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92:1218-1221.