Human Beta-2 microglobulin (B2M) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. Other fluids not optimized but may be tested.

KitContents

1. Monoclonal Anti-human B2M coated microwell strip plate (96 wells), Cat. # 0021 1 plate 2. Human B2M Std. A, 0 ng/mL, 50 ml Cat. # 0022 1 bottle 3. Human B2M Std. B, 2.5 ng/mL, 0.5 ml, Cat. # 0023 1 Vial 4. Human B2M Std. C, 5 ng/mL, 0.5 ml, Cat. # 0024 1 Vial 5. Human B2M Std. D, 20 ng/mL, 0.5 ml, Cat. # 0025 1 Vial 6. Human B2M Std. E, 50 ng/mL, 0.5 ml, Cat. # 0026 1 Vial 7. Human B2M Std. F, 150 ng/mL, 0.5 ml, Cat. # 0027 1 Vial 8. Anti-B2M-HRP Conjugate, 11 ml, Cat. # 0028 1 bottle 9. Wash Buffer (20X); 50 ml, Cat# 0029 1 bottle 10. HRP substrate Soln A, Cat. # 20SA 1 bottle 11. HRP substrate Soln B, Cat. # 20SB 1 bottle 12. Stop solution, 10 ml, Cat. # T-10 1 bottle 13. Instruction Manual, M-0020 1

Form

Stds=0-150 ng/ml ; Sample=10 ul; Sensitivity= 0.5 ng/ml; 90 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

Human Beta-2 microglobulin (B2M) ELISA Kit crossreactivity with B2M from other species was determined by testing sera from various species. Bovine, Cat, Dog, Donkey, Hamster, Horse, Mouse, Rat B2M showed no significant crossreactivity was detected using 1:5 diluted sera. At 1:5 dilution monkey sera yielded values equal to or higher than the equivalent human serum. It is concluded that the human kit has equal reactivity with the monkey beta-2 microglobulin. Due to the lack of purified B2M or known standards, it is not possible to determine absolute crossreactivity.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates