Human BACE-1 Protein, His Tag

Source : 

Human BACE-1, His Tag (BA1-H5220) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1).

 

Molecule : BACE-1

 

Synonyms : BACE1,ASP2,BACE,FLJ90568,HSPC104,KIAA1149,Memapsin-2

 

Format : Powder

 

Category : Enzymes

 

Accession : NP_036236.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 49.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49.3 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I  integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.

 

References :

(1) Willem M, et al., 2006, Science 314 (5799): 664–6.
(2) Zacchetti D, et al., 2007, Neurodegener Dis. 4: 117-126.
(3) Zhao J. et al., 2007, J. Neurosci. 27: 3639- 3649.