human b-Nerve Growth Factor
- 貨號 Cat. No.
- N-245
- 規格 Size
- 5 μg 10 μg 25μg 50μg 100μg
Description
human b-Nerve Growth Factor
hb-NGF
| Product#: | N-245 |
| Sizes: |
| 5 mg |
| 10 mg |
| 25 mg |
| 50 ug |
| 100 ug |
| Preparation: |
| hb-NGF is a recombinant growth factor produced in E. coli. |
| M.W.: |
| 13.5 kDa for the monomer; in solution hb-NGF is found as a dimer.5 |
| Purity: |
| The purity is greater than 98% pure as estimated by SDS-PAGE and amino acid analysis. |
| Bioassay: |
|
 | Survival of PC12 cells: PC12 cells were grown in the absence of serum and collagen,
|
 | Neurite outgrowth in PC12 cells. PC12 cells were grown on collagen coated 24 well plates in DMEM medium supplemented with 6% fetal calf serum and 6% horse serum for 24 h. hb-NGF (#N-245) was addded at the indicated concentrations. The culture media was changed every 2-3 days. The development of neurites over a period of 5 days was visualized by Methylene Blue staining. |
| Locus Link ID: | Accession # : BC032517 |
| References: |
1. | Roux, P.P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | Levi-Montalcini, R. (1966) Harvey Lect. 60, 217. |
3. | Farinas, I. et al. (1998) Neuron 21, 325. |
4. | Levi-Montalcini, R. et al. (1996) Trends Neurosci. 19, 514. |
5. | McDonald, N.Q. et al. (1991) Nature 354, 411. |
6. | Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. |
7. | Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335. |
8. | Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433. |
9. | Kawamoto, K. and Matsuda, H. (2004) Prog. Brain. Res. 146, 369. |
10. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a
family of soluble, basic growth factors which regulate neuronal development,
maintenance, survival and death in the CNS and the PNS.1 NGF, the first
member of the family to be discovered, was originally purified as a factor
able to support survival of sympathetic and sensory spinal neurons in culture.2
It is synthesized and secreted by sympathetic and sensory target organs and
provides trophic support to neurons as they reach their final target.3
Neurotrophin secretion increases in the nervous system following injury.
Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF
constitutively, however direct trauma and induction of cytokines combine to
increase neurotrophin production in these cells after injury.4
The structural hallmark of all the neurotrophins is the characteristic
arrangement of the disulfide bridges known as the cysteine knot,
which has been found in other growth factors such as PDGF.5
There is a 95.8% homology between the rat and mouse forms, and a
85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function
and plasticity.6 Recently, involvement of NGF in processes not involving
neuronal cells has been shown, such as asthma,7 psoriasis8 and wound healing.9
The biological effects of NGF are mediated by two receptors: TrkA, which is specific
for NGF, and p75NTF, which binds all the neurotrophins.10
Alomone Labs is pleased to release human b-NGF (#N-245) which effectively replaces product #N-235.
This new product has been tested and show to have at
least the same purity and biological activity as product #N-235.
The production process has been changed, and we are now able to offer an
additional two new sizes for this widely used growth factor at a very attractive price