Human AMPK (G1/B2/A2) Heterotrimer Protein_CT006-H0907B

分子 : PRKAG1 & PRKAB2 & PRKAA2

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

纯度: > 90 % as determined by SDS-PAGE

内毒素 : < 1.0 EU per μg of the protein as determined by the LAL method

預測N端 : Met & Met & His

缓冲液 : Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4

NP號 : P54619 & Q9Y478 & P54646

氨基酸序列 : Met1-Pro331 & Met1-Ile270 & Met1-Arg552

標籤 : N-GST & N-His