Human Alpha-Synuclein Protein, His Tag

Source : 

Human Alpha-Synuclein, His Tag (ALN-H52H8) is expressed from E.coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1).

 

Molecule : Alpha-Synuclein

 

Synonyms : SNCA,NACP,PARK1,alpha-Synuclein

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_000336.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 16.3 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 100 mM NaAC, pH7.0

 

Description :

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

 

References :

(1) Butler B, et al. 2015. J Biol Chem. 290(49):29542-54.
(2) Logan T, 2017. Nat Neurosci. 20(5):681-689.
(3) Huang CC, et al. 2018. J Cell Sci. 131(23).
(4) Burré J, et al. 2010. Science. 329(5999):1663-7.