Human Alpha Fetoprotein (AFP) ELISA Kit

SpeciesReactivity

Human

Samples

Serum. AFP in culture medium has also been used (see published references). Plasma, Culture medium and other fluids may be adapted

KitContents

1. Anti-hAFP coated microwell strip plate (96 wells). Ready-to-use 501 2. AFP Standard A, 0 IU/ml/Sample. Diluent, 11 ml 502 3. AFP Standard B, 3 IU/ml; 0.50 ml 503 4. AFP Standard C, 10 IU/ml; 0.50 ml 504 5. AFP Standard D, 25 IU/ml; 0.50 ml 505 6. AFP Standard E, 100 IU/ml; 0.50 ml 506 7. AFP Standard F, 200 IU/ml; 0.50 ml 507 8. Anti-hAFP-HRP Conjugate, 11 ml 508 9. HRP substrate Soln A, 11 ml 500SA 10. HRP substrate Soln B, 11 ml 500SB 11. Wash Buffer (20X), 50 ml WB-20 12. Stop solution, 10 ml T-10 13. Complete Instruction Manual M500

Form

Stds=0-200 IU/ml ; Sample=25 ul; Sensitivity= 1.0 IU/ml; 75 min assay; Quantitative, Sandwich, 96 tests

SpCrossreactivity

Human AFP ELISA kit is specific for human AFP with no significant reactivity albumin or other serum proteins. Human AFP ELISA kit species crossreactivity with AFP from other species (mouse, rat, and monkey) has not been established.

MSDS

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates