Human Adiponectin/ACRP30/apM-1 ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Adipocyte; C1q and collagen domain-containing protein; ADIPOQ; Adipocyte complement-related 30 kDa protein; ACRP30; ACDC; ACRP30; APM1; GBP28;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1428

 

Description (Long Description) : 

The human Adiponectin Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human Adiponectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Adiponectin concentration are added to these wells and allowed to complex with the bound Adiponectin antibody. A biotinylated anti-human Adiponectin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Adiponectin present in the samples. The purpose of this kit is for the quantitative detection of Human Adiponectin in cell culture supernatants, serum, heparin or EDTA treated plasma, and urine when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Assay immunogen and standard is recombinant human adiponectin amino acids 19-244 expressed in and purified from a mammalian cell expression system

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :