Human ADAM15 ELISA Pair Set

分子 : ADAM15

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 46.88-3000

 

包被抗體 : 1.0 mg/mL of rabbit anti-ADAM15 monoclonal antibody(in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10517-R027)

 

檢測抗體: 0.5 mg/mL rabbit anti-ADAM15 monoclonal antibodyconjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10517-R007)

 

標準品 : Each vial contains 96 ng of recombinant ADAM15. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of Human ADAM15 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The Human ADAM15 ELISA Pair Set is for the quantitative determination of Human ADAM15.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for ADAM15 coated on a 96-well plate. Standards and samples are added to the wells, and any ADAM15 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-ADAM15 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of ADAM15 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

 

檢測限: 47 pg/ml

 

血清樣本檢測範圍 :