Human ACE ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; Kininase II; CD143;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1419

 

Description (Long Description) : 

The human ACE Kit is a sandwich ELISA. The capture antibody is a¶Ã¿monoclonal human ACE antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ACE concentration are added to these wells and allowed to complex with the bound ACE antibody. A biotinylated goat anti-human ACE polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ACE present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ACE in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Angiotensin-converting enzyme (ACE) converts angiotensin I to angiotensin II.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :