Human a-Thrombin (Factor IIa)

Prepared from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Human Thrombin is homogeneous as judged by 10% SDS-PAGE gels. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin. Buffer composition = 50 mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5 *Extinction Coefficient (1%) = 18.3 Molecular Weight = 37,000 daltons.