HSV-2 G Strain Quantitated Viral DNA

Virus:  HSV-2, G strain

Virus Preparation: Purified virus

Cell Line for Propagation:  Vero

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

DNA Copy Number:  1-2 × 10⁴ copies/µL (varies by lot, please contact ABI for details)

PCR Analysis of DNA Control: PCR analysis was performed on purified HSV-2_G DNA using the PE Biosystems GeneAmp^® Gold PCR Reagent Kit and primers specific to the DNA polymerase gene of HSV-2. The reaction produced a 391 bp fragment.

DNA Quantitation: Viral DNA copy number is determined by real time PCR1,2 using the Roche LightCycler^®. DNA copy number may vary dependent on the quantitation method used.

Application: ABI’s quantitated DNA controls are prepared from virus, bacteria, parasites, or mollicutes, and are intended for use as positive PCR quantitation standards for the organism in question.  Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls.  Additionally, due to the extreme sensitivity of detection in PCR reaction, and since no method of purification can guarantee the complete absence of extraneous agents, DNA controls are not intended for use as negative controls for other organisms.