HSV-1 MacIntyre Strain Purified Viral Lysate

HSV-1, strain MacIntyre purified virus lysed with Triton X-100. Virus is completely inactivated and contains all viral proteins present in active preparation. Can be used for applications such as ELISA or Western blot requiring non-infectious viral proteins.

Virus: HSV-1, MacIntyre strain

Virus Preparation: Purified virus

Cell Line for Propagation: Vero

Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5

Preparation of Lysate: HSV-1 purified virus adjusted to a protein concentration of approximately 1.0 mg/mL and Triton X-100 added to a final concentration of 0.5 % in TNE buffer.

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Virus Particle Count: Varies by lot.

Functional Activity: Reactive in Western blot. Reactive in ELISA using HSV-1 gG MAb.

Final Fill Volume: Varies by lot

Validation of Inactivation: These lots of viral lysate has been specifically tested for residual infectivity and no infectious virus was detected. The lysate was titered in Vero vells over a 7-day period with endpoint determination by cytopathic effects (CPE). This product has been treated using methods of inactivation that have been validated in vitro for complete inactivation of high-titered herpesviruses.