HSPG2 Antibody (LS-B2468)

貨號 Cat. No.: LS-B2468
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2468
Antibody Validation:	This antibody replaces catalog number LS-C15757. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Heparan Sulfate Proteoglycan 2 (perlecan) (HSPG2)
Synonyms:	HSPG2, basement membrane-specific heparan sulfate proteoglycan core, basement membrane-specific heparan sulfate proteoglycan core protein, hspg, hspg2, perlecan
Host:	HSPG2 antibody was produced in Mouse
Antigen Species:	HSPG2 antibody was raised against Bovine
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Cells
Immunogen:	HSPG2 antibody was raised against bovine corneal endothelial cells.
Antibody Specificity:	Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.
Predicted Species Reactivity:	Human
Purification:	Protein A/G Column
Presentation:	PBS, pH 7.4, 0.09% sodium azide
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2468 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2468 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB) (1:50), Immunoprecipitation (IP), ELISA (1:4000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml