HSPB1 Antibody (LS-B2642)

貨號 Cat. No.: LS-B2642
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2642
Antibody Validation:	This antibody replaces catalog number LS-C24126. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Heat Shock 27kDa Protein 1 (HSPB1)
Synonyms:	HSPB1, CMT2F, DKFZp586P1322, heat shock 27 kd protein, HMN2B, HS.76067, Hsp25, Hsp27, HSP28, SRP27
Host:	HSPB1 antibody was produced in Rabbit
Antigen Species:	HSPB1 antibody was raised against Mouse
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	HSPB1 antibody was raised against synthetic phosphopeptide derived from a region of mouse HSP25 that contains serine 86.
Antibody Specificity:	Reactivity: Mouse HSP25. Endogenous human HSP27 phosphorylated at serine 86 (HeLa cells treated with TNF- ) was weakly detected by this antibody.
Antigen Modification:	pSer86
Predicted Species Reactivity:	Human
Purification:	Immunoaffinity Chromatography
Presentation:	PBS (without Mg2+ an d Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2642 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2642 was determined to be 1:100. Positive control: NIH3T3 cells with and without anisomycin.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:1000), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl