Hsp70 Antibody
- 貨號 Cat. No.
- 3096-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongHsp70/p
pbHost: /bMouse/p
pbAntibody Type: /bMonoclonal/p
pbIsotype: /bMouse IgG1/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bRecombinant human Hsp70/p
pbAccession#: /bAAA02807/p
pbGene ID: /b51095054/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/mk/ht/rb/bv/p
pbApplication: /bWB/IP/IHC/p
pbApplication amp; Usage: /bWestern blot analysis (1 micro;g/ml), immunoprecipitation (5-10 micro;g/ml), immunohistochemistry (5-20 micro;g/ml, frozen amp; paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73)./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bHsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell./p
pbHost: /bMouse/p
pbAntibody Type: /bMonoclonal/p
pbIsotype: /bMouse IgG1/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bRecombinant human Hsp70/p
pbAccession#: /bAAA02807/p
pbGene ID: /b51095054/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/mk/ht/rb/bv/p
pbApplication: /bWB/IP/IHC/p
pbApplication amp; Usage: /bWestern blot analysis (1 micro;g/ml), immunoprecipitation (5-10 micro;g/ml), immunohistochemistry (5-20 micro;g/ml, frozen amp; paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73)./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bHsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell./p