Hsp70 Antibody
- 貨號 Cat. No.
- 3097-100
- 規格 Size
- 100 µg
Description
pnbsp;strongAntibody Target :/strongHsp70/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 559 of human Hsp70/p
pbAccession#: /bP5462/p
pbGene ID: /b3306/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/mk/ht/rb/bv/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blot analysis (1-2 micro;g/ml). However, the optimal conditions should be determined individually. The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73)./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bHsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress./p
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pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 559 of human Hsp70/p
pbAccession#: /bP5462/p
pbGene ID: /b3306/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/mk/ht/rb/bv/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blot analysis (1-2 micro;g/ml). However, the optimal conditions should be determined individually. The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73)./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bHsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress./p
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