HSP 60 (T547) Antibody

貨號 Cat. No.

BS1179

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

 

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

HSP60/HSPD1 (T547) antibody detects endogenous levels of HSP60/HSPD1 protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF:1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of HSP60/HSPD1 (T547) antibody in extracts from COLO205 cells.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of HSP60/HSPD1 (T547) antibody in paraffin-embedded human lung carcinoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

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