HSF1 Antibody (LS-B2682)

貨號 Cat. No.: LS-B2682
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2682
Antibody Validation:	This antibody replaces catalog number LS-C7378. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Heat Shock Factor Protein 1 (HSF1)
Synonyms:	HSF1, heat shock factor protein 1, HSTF1
Host:	HSF1 antibody was produced in Rabbit
Antigen Species:	HSF1 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Recombinant protein
Immunogen:	HSF1 antibody was raised against recombinant human HSF-1 expressed in E. coli
Antibody Specificity:	Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, bovine, canine, chicken, Drosophila, gerbil, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.
Predicted Species Reactivity:	Monkey, Mouse, Rabbit, Rat
Purification:	Protein A Column
Presentation:	PBS, 0.09% sodium azide, 50% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2682 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2682 was determined to be 5 ug/ml. Western Blot: Positive control: Recombinant Human HSF-1 Protein.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:500) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml