Proteins and Peptides
HNRNPM / HNRPM Antibody (LS-B2427)
- 貨號 Cat. No.
- LS-B2427
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2427 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C14203. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | HNRNPM / HNRPM |
| Synonyms: | HNRNPM Antibody, DKFZp547H118 Antibody, HnRNP M Antibody, HNRNPM4 Antibody, HNRPM Antibody, HNRPM4 Antibody, HTGR1 Antibody, NAGR1 Antibody |
| Host: | HNRNPM antibody was produced in Rabbit |
| Antigen Species: | HNRNPM antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | HNRNPM antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa161-182 [MATTGGMGMGPGGPGMITIPPS] of human carcinoembryonic antigen receptor "long" (CEARL) |
| Antibody Specificity: | Recognizes human CEARL/hnRNP M4 isoform 1, Mr ~74-80kD. Additional proteins were also detected, Mr ~55 and 70kD. Species cross-reactivity: Mouse and rat. |
| Antigen Modification: | aa161-182 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Protein A Column |
| Presentation: | 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30% |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2427 was determined to be 5 ug/ml. Western Blot: 0.1-0.5 ug/ml Detects CEARL in RIPA lysates from HeLa cells. Immunocytochemistry: 1:1000. Reported to show positive immunostaining for CEARL in rat Kupffer cells. FACS: Reported to detect CEARL in rat Kupffer cells by FACS analysis. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC) (1:1000), Western blot (WB) (0.1 - 0.5 µg/ml), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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