hNeurotrophin-4
- 貨號 Cat. No.
- N-270
- 規格 Size
- 1 μg 5x1 μg 5 μg EconoSize! 10 μg
Description
hNeurotrophin-4
(NT-4)
| Product#: | N-270 |
| Sizes: |
| 1 μg |
| 5 x 1 μg |
| 5 μg |
| EconoSize! 10 μg |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family
of soluble, basic growth factors which regulate neuronal development, maintenance,
survival and death in the CNS and the PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons
of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is
synthesized by keratinocytes.4
The structural hallmark of all the neurotrophins is the characteristic arrangement of the
disulfide bridges known as the cysteine knot, which has been found in other
growth factors such as PDGF.5
The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote
dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6
to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde
survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9
The biological effects of NT-4 are mediated by two receptors: TrkB which is specific for NT-4 and
BDNF, and p75 which binds all the neurotrophins.10
| Preparation: |
| hNT-4 is a recombinant growth factor produced in E. coli. |
| M.W.: |
| 28 kDa (monomer 14 kDa, 130 amino acids). |
| Purity: |
| Greater than 98% pure as estimated by SDS-PAGE and HPLC analysis. |
| Bioassay: |
| The activity was determined by the dose-dependent induction of choline acetyl transferease activity in rat basal forebrain primary septal cultures. The ED50 in this assay was found to be in the range of 20-50 ng/ml. |
| Locus Link ID: | 4909 Neurotrophin-4. |
| References: |
1. | Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | Roosen, A. et al. (2001) Prog. Neurobiol. 21, 203. |
3. | Moalem, G. et al. (2000) J. Autoimmun. 15, 331. |
4. | Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515. |
5. | McDonald, N.Q. et al. (1991) Nature 354, 411. |
6. | DeFazio, R. A. et al. (2000) Neuroscience 99, 297. |
7. | Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56. |
8. | Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485. |
9. | Lobner, D. and Ali, C. (2002) Brain Res. 954, 42. |
10. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35 |