hNeurotrophin-3 (NT-3)
- 貨號 Cat. No.
- N-260
- 規格 Size
- 1 μg 5x1 μg 5 μg EconoSize! 10 μg
Description
hNeurotrophin-3 (NT-3)
| Product#: | N-260 |
| Sizes: |
| 1 μg |
| 5 x 1 μg |
| 5 μg |
| EconoSize! 10 μg |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family
of soluble, basic growth factors which regulate neuronal development, maintenance,
survival and death in the CNS and the PNS.1 Of all the neurotrophins, Neurotrophin-3 (NT-3) shows
the highest expression levels during the perinatal development, with most prominent
expression levels in the hippocampus, the neocortex and the cerebellum.2
Release of endogenous NT-3 was observed from cultures of embryonic hippocampus
following high K+ induced depolarization or stimulation with activity dependent
neurotrophic factor (ADNF).3
The structural hallmark of all the neurotrophins is the characteristic arrangement of
the disulfide bridges known as the cysteine knot, which has been found in other
growth factors such as PDGF.4 The rat and human forms of NT-3 are 96% homologous.
NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,5
to serve as an anti-inflammatory factor to suppress microglial activation,6
to play a critical role in regulating T helper 1/T helper 2 cell balance7
and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.8
The biological effects of NT-3 are mediated by two receptors:
TrkC, which is specific for NT-3 and p75, which binds all the neurotrophins.9
| Preparation: |
| hNT-3 is a recombinant growth factor produced in E. coli. |
| M.W.: |
| 28 kDa (monomer 13.6 kDa, 119 amino acids). |
| Purity: |
| Greater than 98% pure as estimated by SDS-PAGE and HPLC analysis. |
| Bioassay: |
| The activity was determined by the dose-dependent induction of choline acetyl transferease activity in rat basal forebrain primary septal cultures. The ED50 in this assay was found to be in the range of 20-50 ng/ml. |
| Locus Link ID: | 4908 Neurotrophin-3. |
| References: |
1. | Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | Zhou, X.F. and Rush, R.A. (1996) Brain Res. 643, 162. |
3. | Blondel, O. et al. (2000) J. Neurosci. 20, 8012. |
4. | McDonald, N.Q. et al. (1991) Nature 354, 411. |
5. | Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. |
6. | Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. |
7. | Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221. |
8. | Kimitsuki, T. et al. (2003) Auris. Nasus Larynx. 30, 141. |
9. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. |