hLIF

hLIF

human Leukemia Inhibitory Factor

Product#:

L-200

Sizes:

1 mg

5 x 1 mg

5 mg

Leukemia inhibitory factor (LIF) was identified by its ability to induce terminal differentiation 

in leukemic cells.^1,2 LIF is a pleiotrophic factor with known actions in the immune system,  

the nervous system and the reproductive system.^3,4 

In the nervous system it acts on cultured sympathetic neurons to direct a change in neurotransmitter 

expression from a noradrenergic to a cholinergic phenotype and regulates the

expression of neuropeptide transmitters in these cells.^5,6 

 

In the immune system LIF plays a key role in inflammation,^7,8 with a pro-inflammatory role 

in rheumatoid arthritis,⁹ but also with proposed anti-inflammatory properties  

in lung inflammatory processes. 

 

In the reproductive system, LIF appears to play an important role in implantation 

and in the establishment of pregnancy.^10,12 LIF acts as a trophic factor for  

oligodendrocytes and promotes astrocytic survival and differentiation.^13,14 It exhibits activity 

towards spinal motor neurons and stimulate the biosynthesis of acetylcholine.⁶  

LIF also functions as a trophic factor for peripheral sensory neurons  

supporting their survival.^15,16 LIF appears to be essential for injury-induced  

neuropeptide synthesis¹⁷ and can also stimulate the hypothalamic-pituitary-adrenal axis  

in response to stress and disease.¹⁸ LIF is used extensively in experimental biology 

because of its ability to induce embryonic stem cells to retain their totipotentiality.

Preparation:

hLIF is a recombinant neuropoietic cytokine produced in E. coli.

M.W.:

19.7 kDa (180 aa residue glycoprotein).

Purity:

Greater than 98% as estimated by SDS-PAGE analysis.

Bioassay:

The biological activity was confirmed by its ability to activate ERK1/2 and STAT3 protein.

LIF triggered ERK and STAT activation. 3T3-L1 cells were serum starved for 2h and stimulated with 100ng/ml hLIF (#L-200) for 10 min. The cell proteins were resolved by SDS PAGE and detected with anti-phospho-STAT3 (upper panel) or with anti-phospho-ERK1/2 (lower panel).

Locus Link ID:

3976 (Leukemia Inhibitory Factor).

References:

1.	Brach, M.A. et al. (1990) Leukemia 4, 646.
2.	Estrov, Z. et al. (1992) Leuk. Lymphoma 8, 1.
3.	Gough, N.M. et al. (1989) Cancer Cells. 1, 77.
4.	Metcalf, D. et al. (2003) Stem Cells 21, 5.
5.	Zurn, A.D. et al. (1994) Dev. Biol. 163, 309.
6.	Martinou, J.C. et al. (1992) Neuron 8, 737.
7.	Gadient, R.A. et al. (1999) Stem Cells 17, 127.
8.	Patterson, P.H. (1994) Proc. Natl. Acad. Sci. U S A. 91, 7833.
9.	Gabay, C. et al. (1996) Clin. Exp. Immunol. 105,260.
10.	Lass, A. et al. (2001) Fertil. Steril. 76, 1091.
11.	Robb, L. et al. (2002) J Reprod. Immunol. 57, 129.
12.	Ledee-Bataille, N. et al. (2002) Hum. Reprod. 17, 213.
13.	Vos, J.P. et al. (1996) Perspect. Dev. Neurobiol. 4, 39.
14.	Butzkueven, H. et al. (2002) Nat. Med. 8, 613.
15.	Horton, A.R. et al. (1996) Perspect. Dev. Neurobiol. 4, 35.
16.	Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355.
17.	Rajan, P. et al. (1995) Neuroreport 6, 2240.
18.	Akita, S. et al. (1996) J. Clin. Endocrinol. Metab. 81, 4170.