HIV-1 IIIB Strain gp160 env Purified Native Glycoprotein

貨號 Cat. No.
14-103-050
規格 Size
50 µg
品牌 Brand
Advanced Biotechnologies Inc

Description








Catalog Number: 

14-103-050






Status: 

Current product






Availability: 

Usually ships within two business days






Size: 

50 µg






Use: 

For research use only. Not for use in diagnostic procedures.






Storage Temperature: 

-70ËšC




Product Description: Soluble native gp160 from HIV-1IIIB infected H9 cells. This oligomeric, fully glycosylated native viral protein is isolated from conditioned cell culture medium inactivated with 0.5% Triton X-100 and purified by immunoaffinity chromatography using a mouse monoclonal antibody to HIV-1 gp41.


Protein Concentration: [varies by lot] µg/ml determined by BCA protein assay method using BSA standard. Alternative methods for determining protein concentration may give different results.


Final Fill Volume: [varies by lot]


Presentation: Purified native glycoprotein, gp160 of HIV-1IIIB, is supplied in PBS and is typically oligomeric.


Purity: 95% pure as estimated by analysis of Coomassie blue stained SDS- PAGE.


Functional Activity: Immunoreactive in ELISA1 and Western blot using HIV-1 antibody positive human sera. For ELISA, 10-100 ng protein and for Western bot, 100ng - 2 ?g protein is recommended depending on the nature and affinity of the detection reagent.






Shipping and Storage: 

This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.



 






Safe Handling Recommendations: 

This product has been produced and purified in the absence of infectious virus preparation. However, the protein is biologically active and could potentially generate a protein-specific immune response if an exposure incident






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