Proteins and Peptides
Histone H4 Antibody (LS-B2036)
- 貨號 Cat. No.
- LS-B2036
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2036 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C695. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Histone H4 |
| Host: | Histone H4 antibody was produced in Rabbit |
| Antigen Species: | Histone H4 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | Histone H4 antibody was raised against synthetic peptide |
| Antibody Specificity: | A synthetic peptide containing amino acid residues 15-30 of human Histone H4 GAKRHRKVLRDNIQGI. This sequence is conserved across mammalian, invertebrate and plant species. An NCBI BLAST shows no cross-reactivity of this sequence with other known histones. |
| Antigen Modification: | aa15-30 |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.02% sodium azide. |
| Recommended Storage: | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2036 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2036 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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